Translational_Unit

Part:BBa_K2029012:Design

Designed by: Bui Hoang Duy   Group: iGEM16_UBonn_HBRS   (2016-10-14)


nprb_CelA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for use in Bacillus subtilis, C-terminal 6His-tag added for convenient purification


Source

Enzyme coding gene: https://www.ncbi.nlm.nih.gov/pubmed/22101447

References

https://parts.igem.org/Part:BBa_K2029000 https://parts.igem.org/Part:BBa_K2029004